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Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Dilute to 1X with dH2O. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal.

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Bring to ml with deionized H It should be noted that for the best possible results a fresh blot is always recommended. Drain membrane of excess developing solution. Blotting Membrane and Paper:

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